Genetic tool to be applied to unidentified bodies in Colombia
Abstract
The purpose of this research is to contribute to the understanding of new tools to be applied in unidentified bodies based on STRs genetic markers, which have a high discrimination power, observed heterozygosity higher than 70%; distant chromosomal locations or, ideally, in different chromosomes to ensure independent segregation; robustness and reproducibility of results in multiplex PCR. In addition to a PCR fragment yield of less than 500 base pairs to achieve better performance in samples with degraded DNA. Additionally, other variables are analyzed, such as Indels classification, the allelic frequency distribution of Indels markers, FST fixation indexes, and allelic frequency distribution for the Central Andean Region, among others. Among the most relevant conclusions of the study is the creation of reference databases from different regions of the country for a panel of 38 Indels markers of easy characterization using a technique of simple implementation in all existing forensic laboratories.
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