Genetic diversity analysis of 21 Moniliophthora roreri fungus’ isolations based on RAPD markers
Abstract
Objectives: To study the genetic diversity of 21 fungus’ isolations that affect cocoa crops, Moniliophthora roreri in three cocoa areas (cacaoteras) from Peru (Tocache, Mariscal Cáceres and Leoncio Prado). Methods: 14 polymorphic RAPD indicators (random amplified polymorphic DNA) and an oligonucleotides pair were used, which were employed under standardized amplification terms. With the obtained data a dendrogram was constructed using the Jaccard coefficient and the UPGMA (Unweighted Pair-Group Method using Arithmetic Average) algorithm. The genetic structure was estimated based on the variance molecular analysis (AMOVA) and the diversity based on the Shannon and Nei indices. Results: We collected 59 RAPD bands with 73% of polymorphism. The obtained dendrogram at a similarity index of 0,70, it clearly divided the individuals in three groups. The genetic diversity analysis showed high values in the three studied areas according to the Shannon index (0,3936) and Nei (0,2622), more richly in Leoncio Prado. The three areas have high variability within them, according to the performed AMOVA: 88% between accessions by area and only 12% between areas. Conclusions: The existence of more than one genetic group of Moniliophthora roreri in the Peru’s Amazonia arises. These groups from Ecuador, may have entered by the exchange of seeds and/or in a natural way by rivers in common and they would be originating new local genetic groups.References
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